What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to analyze dendritic spines from tissue (Prelimbic cortex)?

Hi there, I am trying to measure dendritic spines from mouse fixed tissue, specifically, I am looking at the prefrontal cortex. I injected the PrL with a lentivirus to knock-down my protein of...

27 January 2021 9,251 2 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

25 January 2021 3,587 3 View

How can I carry out analysis of pulp and paper properties according to Tappi standard method in the Lab?

Properties such as relative humidity (T 402sp-03), brightness and yellowness (T-452 om-02), whiteness (T-560 pm-96), opacity and absorption coefficient (T-425 om-01), tensile, breaking length and...

24 January 2021 308 3 View

PROCESS V3.5.2 macro for SPSS variable name length limitations?

I just installed PROCESS V3.5 in SPSS and am receiving the follow error when I try to run a model: ERROR: Variable names should be no more than eight characters in length. I don't recall...

11 January 2021 3,448 4 View

What is the kinetics that Cr6 on the surface of the soil will preferably exchange with the charge on the surface of the iron oxide nanoparticle?

I am researching the application of iron oxide nanoparticle in the removal of heavy metal (Cr6) from soil

10 January 2021 3,167 3 View

Can anyone direct me to where I can find the X-Ray elastic constants for WC-Co or just WC?

I have searched all over the internet and I seem not to be arriving at its value. I need this value to perform some XRD measurements. Any help would be greatly appreciated.

05 January 2021 916 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Can you mention any source which presents an updates forest-fire hazard or vulnerability map of North-East India?

There exists a confusion of generating fire-hazard maps with MODIS data as that way, we cannot distinguish between shifting agriculture and wildfires. I am looking for a source that provides me...

22 February 2021 9,616 2 View