Can I use alpha amylase enzyme from (fungal source) if I need to characterize alpha amylase inhibitor from marine algae for hyperglycemia management?

I need to characterize alpha amylase enzyme from macroalgae for management of blood glucose level in diabetic patients, my question is can I use (fungal alpha amylase enzyme) in inhibition assay?

18 January 2021 735 4 View

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

09 January 2021 9,320 3 View

How to design manholes of a stormwater tunnel?

I am exploring for the design code or manual and standard drawings of access and maintenance manholes of the storm water conveying tunnels. (i.e free flow tunnels not pressurized ones) but all of...

22 December 2020 2,590 4 View

How to use TransDecoder for transcriptome analysis?

I am searching for new antimicrobial peptides and I don't have many skills in bioinformatics, so I want to know how to use these software packages to identify candidate coding regions within...

01 December 2020 4,632 3 View

Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

SAS code for a "Split-plot in time" (based on CRD design)?

Dear all, I want to analyze a factorial split-plot in time using SAS. Factorial Experiment using Completely Randomized Design (CRD); Factor A: treatments (a1-a4) Factor B: harvest time,...

28 October 2020 3,515 4 View

Is there a solvent for blending PHB and Acid Ascorbic (via solution casting)?

Dear Researchers I want to add Ascorbic Acid (AA) to PHB in order to improve its biodegradability. AA is soluble in water but insoluble in those that are compatible with PHB (THF, Chloroform,...

07 October 2020 1,136 4 View

Journal vs conference publications, which one would you prefer ?

For sure journal publications have more weight than conference publications but i am asking about your preference. For example i like conferences a lot because i like to meet people and explore...

06 September 2020 7,468 42 View

What is best software for simulation of microfluidic?

I'm working on a microfluidic project. I need a software to simulate all forces include: centrifugal (surely Euler and Coriolis too), and surface tension. Solidworks follow simulation doesn't...

30 August 2020 4,671 9 View

Do ammonium glutamate considered as necessity in salt sloution for Ecoli-based Cell free protein synthesis platform ?

Hello, I am constructing an E-coli based Cell free protein system (CFPS) reagent, but I can't find ammonium glutamate from the local suppliers or from MP biochemical local disruptors. Can I...

13 August 2020 3,691 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View