Hi,

I am working on the conjugation of reduced graphene oxide (rGO) to a polyclonal antibody with an affinity to my E. coli K12 (red fluorescent-tagged). I am designing an experiment to investigate if the conjugation of rGO and Ab is successful or not. Towards this goal, I prepare the rGO-AB sample and then add bacteria to it. I was wondering if I could pellet potential rGO-Ab-Bacteria and discard the excessive rGO-Ab and Bacteria, I might be able to confirm the successful making of the rGO-Ab-Bacteria complex with fluorescent imaging. Is there a centrifugation configuration that could help me? Or is there any other process or experiment that could help me confirm this?

Thanks in advance.

Best regards,

Mohammad