Has anyone experienced problems with cryosectioned tissues? I cryosectioned the mouse brain tissue, and the sections appeared spongy after cutting, which makes them unsuitable for IHC applications. Unfortunately, remodeling or re-preparing the tissues is not an option. I have already incubated the sections overnight in PBS and citrate buffer, but neither has improved the situation. Another issue is that the tissues show strong autofluorescence and unclear cellular morphology.

I would greatly appreciate any advice on approaches for rehydration or other methods that could help reduce or prevent this spongy texture and improve tissue quality for downstream analysis.