I am reading one image and used the same as a reference image as program given below , but if I am using seven frames of video sequence, and need to correlate with reference image , what modification i need to do?

looking forward for your reply !!!

image=imread('image.JPG');

image=rgb2gray(image);

imagecube=zeros(size(image,1),size(image,2),3);

imagecube(:,:,1)=image;

imagecube(:,:,2)=image;

imagecube(:,:,3)=image;

frames = imagecube

Ref = double(image)