Could anyone please let me know how to prepare 15N and 13C-labled rice straw? Expectedly how much time it will take to prepare the labeled rice straw. If possible please send me some good protocols.
Thanks in advance
Naseer
It will be extremely expensive, you need to cultivate rice and to include isotope-labeled urea or other fertilizers in your pots.
Calderon thanks for your kind help. Just out of curiosity could you please send me some good reference/protocol.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3573864/pdf/mee30002-0326.pdf
Methods in Ecology and Evolution 2011, 2, 326–332
A simple method for in situ-labelling with 15N and 13C
of grassland plant species by foliar brushing
Birgit Putz, Thomas Drapela Wolfgang Wanek, Olaf Schmidt, Thomas Frank1 and
Johann G. Zaller
This is another paper similar to what Ana has provided. This one involves 15N and 13C labeling of maize plants using a similar approach. You may be able to adapt the method for rice plants.
Susantha thanks for sending me the related article. Yeah we can use this procedure for rice plants.