I have 2133 log2FC values of six treatment (attached in xlsx file). A data arrangement is arranged for the requirements of xlstat. I would like to perform a volcano plot analysis.
I have tried the demo (36 columns of data) of xlstat for omics --> Different expression tool, in which the Parametric test works properly and results 'regular' volcano plot in order to distinguish the differentially expressed genes on that dataset.
My dataset, however, is smaller than above in the demo (six columns of data) and I can not perform Parametric test. The Nonparametric test is allowed only which results an 'irregular(?..)' volcano plot.
Does anybody have idea what do I wrong? Is it possible that my dataset 2133 rows and six columns - does not allow using Parametric test?
Thanks for the answers.
I mean 'irregular' volcano plot as can be seen on the figure atteched.
are you looking to display your data or do a particular analysis? Volcano plots are easy enough to make by hand in excel if all you want to do is look at the distribution of data.
Hi Nicolas, thank you for the answer. I have solved the problem. I used a script and used the 'calibrate' package in R.
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