Hello,

I need to validate/quantify some lipids that i found disregulated in my previous untargeted work. The deuterated standards are very expensive or they not exist, so I've had thought that i can perform a PRM with an inclusion list with m/z and retention times of my lipids.

The method work and I've acquired the MS/MS spectra of my precursors, but now there is the problem: how can I process these data?

For proteomics is simple: I've read that there are a lot of softwares, but for lipids is more complicated. I found some articles (in attachment) where they use skyline, but it is not very clear how they modify the peptide sequence (i'm not an expert .

So here my questions: anyone know a free software where I can perform this kind of quantification? There are other ways/methods to quantify lipids?

Thank you in advance

I work on a Orbitrap Q-exactive Plus (Thermo)