Is there a procedure on how to microencapsulate cells into an alginate gel? I was going to use Trypsin-EDTA to remove, centrifuge, and then add media to get cells. From then, I would add the cell/cell media into the gel via a pipette but with my growth factors to determine a suitable microenvironment.
Any and all help is appreciated. Thanks!
Natural polymers for the microencapsulation of cells
Luca Gasperini, João F. Mano, Rui L. Reis
Abstract
The encapsulation of living mammalian cells within a semi-permeable hydrogel matrix is an attractive procedure for many biomedical and biotechnological applications, such as xenotransplantation, maintenance of stem cell phenotype and bioprinting of three-dimensional scaffolds for tissue engineering and regenerative medicine. In this review, we focus on naturally derived polymers that can form hydrogels under mild conditions and that are thus capable of entrapping cells within controlled volumes. Our emphasis will be on polysaccharides and proteins, including agarose, alginate, carrageenan, chitosan, gellan gum, hyaluronic acid, collagen, elastin, gelatin, fibrin and silk fibroin. We also discuss the technologies commonly employed to encapsulate cells in these hydrogels, with particular attention on microencapsulation.
http://rsif.royalsocietypublishing.org/content/11/100/20140817
Hi Joshuah! How are you? If you want to encapsulate cells into alginate gel you must resuspend cells into the alginate solution after centrifugation. Subsequently, with a solution of Calcium Chloride you can cross-link the alginate solution obtaining the cell-laden gel. I can provide you further details if you give me your email!
Hi Nicola!
Great to hear from you! I apologize for the delay as I have not been very active on Research Gate.
I appreciate your help! I recently sent you my email inquiring about this procedure since I am still a novice at this. Please let me know how this is done.
Thanks!
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