I have MNcs were kept frozen (-80) with FBS and the cells died. Can I measure measure the NFkB (total) in these cells? if it possible, what is the best Kit for this?
I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...
23 July 2024 5,703 0 View
I am currently working on a mendelian randomization study, and I have downloaded the datasets needed from the ieu opengwas project (mrcieu.ac.uk) in .vcf format. I do not have access to an R...
19 July 2024 2,342 5 View
ResearchGate sends me verification mail to incomplete email address. I coculd no find he entry to correct it.
27 February 2024 6,280 1 View
Hello, I am a fifth-year medical student. I want to initiate research in the field of neuroregeneration after injury. However, I am facing difficulties in identifying a starting point for my...
10 August 2023 3,306 1 View
I have prepared SBF many times according to ISO 23317-2014 and none of them gave me the correct ICP results as mentioned in the ISO document. Cl ions are too high while Mg ions are low, as well...
04 October 2020 5,062 0 View
what is the best and simple way to derivatize phenolic compounds to fluorescent derivatives??
09 December 2019 5,844 3 View
Is there any method or protocol to increase the permeability of mouse Zona Pellucida to large molecules of around 100 nm diameter?
03 December 2019 4,265 3 View
Generation of genetically modified mice using CRISPR/Cas9 system requires the delivery of CRISPR reagents into the genome. Some scientists use pronuclear microinjection of CRISPR reagents while...
03 December 2019 171 1 View
I am working on analysis of some phenolic compounds via TLC scanning in fluorescence mode. I noticed a decrease in peak areas upon increasing concentation in all used concentration ranges...
18 October 2019 2,024 3 View
I have extracted RNA from oat, I sent the samples to check the integrity of RNA by bio-analyser. I got more than 28s and 18s bands, people who did the analysis told me that " Then they are quite...
02 April 2019 3,638 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View