I want to analyse S. cerevisiae Chromosome Lenght Polymorphism (CLP). I prepare 1% Clean cut agarose blocks with CHEF Genomic DNA Plug Kit (Biorad) and I run it in 1% agarose using CHEF DR III system. Running conditions are: 60-120 s, angle 120°, 24 h, 14°C. I always get the same results - the bands are blurry (even in case of the ladder - ready-to-use CHEF DNA S. cerevisiae Size Marker). In the enclosed picture first row from the left represents the marker. I have tried different agaroses (PFGE agarose, Biorad; Low Melt agarose, Biorad). Could you please give me any advice on how to improve the sharpness on my gels?