How to characterize a covalent bond?

I am doing silane modification of a glass slide, I would like to find a way to characterize and prove that the silane modification done on glass surface is through covalent bonding not through...

29 May 2019 187 3 View

What is the possible surface coverage of nanoparticles (silica) upon silane functionalization?

I would like to get a general idea on the volume of surface area of any nanoparticles that will react with functionalizing agent considering steric hindrance and other factors.

01 May 2019 8,569 3 View

How to get pore water pressure expulsion from foundation soil only for the embankment weight using ANSYS Mechanical APDL??

I need to know how to get pore water pressure of foundation soil for the applied embankment soil weight using ANSYS Mechanical APDL. Anybody please help me..

17 March 2019 8,823 1 View

Has anyone simulated missile Trajectory in Matlab ?

I. Have to simulate a maneuvering missile Trajectory.. Please let me know if any one has simulated missile Trajectory path in Matlab

19 February 2019 5,289 4 View

How to calculate pH for the system in theoretical calculations, where the number of H+, water molecules and polymer chains in system is known?

I know, pH=-log (H+); where H+ is a conc. of H+ or H3O+ given by molarity (M). To calculate molarity the volume of total system is taken to be 1 L, but for theoretical calculations what is to be...

11 February 2019 1,793 1 View

Can N2 gas used for purging get converted to NH3/NH4 in anammox reactor?

I have set up Batch Anammox reactor. To maintain anaerobic conditions, the reactor is purged with Nitrogen gas. I would like to know whether this nitrogen gas used for purging can get converted to...

26 December 2018 4,347 3 View

Can you please help me?I am also working on wireless sensor network security.I need your help.will U?

I am Ph.D scholar and have just started working on WSN SECURITY few months back. I am really in a need of help

06 December 2018 9,546 3 View

If a dependent variable is binary and independent variables are ordinal, which regression model is appropriate?

I have survey data to find when people continue to use a device or stop using it. The preferences are ordinal values, and the response is binary value of using the device or not. Which statistical...

22 December 2017 9,707 5 View

Has anyone used primers >100bp for cloning? Please help?

I am trying to stitch in a 38 amino acid tag to the N-terminal end of my protein (3200bp) to be cloned into a lentiviral vector (~7000bp). The forward primer for the same, along with the overhang...

25 October 2017 4,308 6 View

What could be done to avoid the disruption in DNA during MD simulation?

01 September 2016 2,669 7 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

What HPLC Marker(s) should be used to detect anthocyanins in a sample?

What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?

23 February 2021 1,832 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which vial septa the right one to be used for HPLC?

This is the unanswered question stored very long in my mind and till now I could not find a right answer. Google search educated me that the red surface is the PTFE material compatible to wider...

21 February 2021 546 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View