you should make DNA:DNA hybridisation with other strain or do GTG5 PCR AND BOX PCR. 

A simple blast for 16s is not a reliable methode for molecular identification, You have to draw a phylogenetic tree with comparison of your sequence with other registered seq. in the NCBI using Mega6 or other softwares.

After that you need to do some supplementary assay on your isolate such as some differential biochemical tests

Hi Jamil,

Just before you continue, ensure that your sequencing data is clean and there is no errors in your sequencing data. Looking at the image you have uploaded, your sequence is ~99% similar to the nearest phylogenetic neighbour according to blast. Chances are that your isolate is most likely the organism blast is suggesting. Note however that anyone can upload sequences to blast, including partial sequences, new strains, etc. and as such blast is not always the best website to use. Might I suggest that if you want to search type strains, obtain sequences, etc. for phylogeny you could try the Ribosomal Database Project: http://rdp.cme.msu.edu/seqmatch/seqmatch_intro.jsp

Simply paste your sequence, and change the options to the following..>

Strain: Type

Source: Isolate

Leave the rest as default. 

This should load a screen with the nearest closest type relative, and from there click "View selectable matches"

This will bring up the type strains, which will provide you with less cumbersome information. You can also take the sequences and generate trees with the data. Feel free to message me if you require additional help.

Hope this helps :) 

Hello Jamil,

I agree with Mitchell. You need to test your sequences against type strains. 

RDP is a good option. I suggest checking EZtaxon (http://www.ezbiocloud.net/eztaxon/identify) as well.

Keep in mind that Firmicutes and Bacillus especially have very problematic phylogeny.

thank you very much Mustafa Ozkan Baltaci, Reza Khakvar, Mitchell Henry Wright and Ray Keren. if I need further help i'll contact to to people 

thank you very much Mustafa Ozkan Baltaci, Reza Khakvar, Mitchell Henry Wright and Ray Keren. if I need further help i'll contact to to people 

Dear Jamil: Reading the previous suggestions of the other colleagues I must say I agree with them. Microbiological, biochemical and molecular characterization using 16S rDNA are like the first common steps. 

There are also available many services for a complete strain characterization at different strain collections which include DNA:DNA hybridization, complete genome sequencing, cellular fatty acid composition, mycolic acid analysis, etc., but they are a little expensive and time consuming.

You should check too the ribosomal database Mitchell adviced you.

Good luck. 

Best wishes, Rolando