How can I identify alternatively spliced variants in a single gene?

12 December 2013 0 10K Report

I am trying to identify splice variants in three seperate genes.

I have tried using primers designed for the UTR regions in order to amplify any variant inside those regions in cDNA. The UTR regions at the moment due to a lack of knowledge about the genes are unknown therefore I am instead designing primers to regions flanking the gene in the gDNA approximately 200bp or less away from the start and stop codons. Is this an appropriate method? Are there any other easy and effective methods?

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