Sam O'Donnell

2 Questions 2 Answers 0 Followers

Questions related from Sam O'Donnell

How can I identify alternatively spliced variants in a single gene?

I am trying to identify splice variants in three seperate genes. I have tried using primers designed for the UTR regions in order to amplify any variant inside those regions in cDNA. The UTR...

12 December 2013 9,663 0 View

How can I isolate alternatively spliced mRNA variants of a specific gene?

I am trying to identify splice variants in a specific gene. Am I able to design primers to the UTR regions in order to capture the internal variation ignoring splice variation at the UTR regions?...

12 December 2013 2,538 2 View