I snapfroze liver tissue in liquid nitrogen without the use of any fixative.
I would now want to stain these with H&E.
Is these a way this can be reliably done without damage to the tissue/introducing artefacts?
To complicate things, some samples are infected with a BSL-2 virus LCMV, which would need to be killed in one way or another before sectioning.
Many thanks!
Hi Zsofia Keszei
H&E staining can be done in frozen tissues, However I do not know of any process to remove the virus (BSL-2 virus LCMV).
If you want to do sectioning with cryotome, prepare the blocks with OCT medium and cut the sections in slides. Following sectioning the slides can be fixed and stained with the protocol attached.
https://mousepheno.ucsd.edu/hematoxylin.shtml
Thanks,
Before you start with you staining fix the tissue sections in 4% PFA pH 7.4 over night in the fridge (4°C). This procedure protect the tiusse and kills the virus. After several rinses in distilled water you can start with your staining precess.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Writing