Terpenoids are very different from the other compounds- phenolics and flavonoids overlap considerably since most flavonoids contain phenolic groups.

I suggest using an ion exchange column (SAX, strong anion exchange) . This will capture your phenolic compounds and the terpenes will pass through the column. you can use Sephadex LH-20 and C18 to purify the phenolic compounds, or a polyamide column.

The terpenes, which won't be retained on the ion exchange column, can be purified with silica column chromatography.

http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN87_RediSep%20Rf_SAX_Column_Applications.pdf

http://www.isco.com/WebProductFiles/Applications/101/Application_Notes/AN98_Hints%20for%20Strong%20Ion%20Exchange%20Resins.pdf

http://www.isco.com/WebProductFiles/Applications/101/Poster_and_Paper_Reprints/Purification_of_Phenolic_Flavonoids.pdf

Hi Vikas,

You can fractional distillate to isolate volatile components in a mixture.

Attached is a link explaining how to do that.

After distillation you may run a short column to purify the isolated components. TLC is a good way to identify how much impurities you have in your sample. Subsequently proton NMR spectroscopy would suggest further clarification of your isolated sample.

http://www.chemguide.co.uk/physical/phaseeqia/idealfract.html

You can dissolve youre extract in methanol 70% (that prepared by distilled water ) and  added petroleum ether into decantor or liquid-liquid extractor and isolate volatile phase from youre extract after shaking of decantor or heating of liquid-liquid extractor. this method cause be isolated terpene compounds from youre mother extract. liquid-liquid extractor can be better fraction youre extract than decantor.  

As you have done the defatting already, so there is no question of any lipids or steroidal molecules in the ethanolic fraction. 

Ethanol will have all your desired compounds. If you have standards, do TLC and find the presence of target compounds in the ethanol extract. If you succeed, try column to separate the particular compound. 

After defatting, the mother liquor is subjected to liquid-liquid fractionation by solvents of increasing polarities viz. CH2Cl2, EtOAc and finalyy by n-buOH. The compounds you are asking for have a wide range of polarities starting from aglycones to monosidesm biosides and more. In Ch2Cl2 you get the aglycones. In EtOAc you may find monosides and in n-buOH wou will get biosides and more. Moreover, the polarity depends also on the number of OH groups in the compound and the type of sugars. I mean if you have deoxy sugars you may find some glycosides in Ch2Cl2 extract and more than biosides in EtOAc extract and so on. There is no specific rule and everything depends on the hydroxylation pattern of your compounds and he number of sugars

Yeah I had done fractionation with chloroform then ethyl acetate and then finally with butanol.Then i check for presence of phenolics flavonoids and terpnoids in all fractions.

But i got problem ..my left out aqueous after butanol fraction is more active as comared to other fractions.Now what should i do?