I suppose that "2R" means the diameter of a powder ring, and you want to find the d-value from 2R.
If the scale of your SAED pattern is not changed by image processing,i.e. if it reflects the true camera length (CL) in mm then simply multiply CL with the wave length of the electrons.
Lambda(Angstroem) = 12.2639/sqrt(hv*(1.+.97845e-06*hv)) hv = high voltage[V, not kV!]
100kV -> 0.0371330 Angstrom
200 -> 0.0250787
300 -> 0.0196870
CL*lambda is the camera constant [CC] in Angstrom*mm, and the scale bar for 1Angstroem**(-1) has the length of CC[mm]
d(Angstrom) = CC/R
I hope my guess concerning the meaning of "2R" was correct.
I suppose that "2R" means the diameter of a powder ring, and you want to find the d-value from 2R.
If the scale of your SAED pattern is not changed by image processing,i.e. if it reflects the true camera length (CL) in mm then simply multiply CL with the wave length of the electrons.
Lambda(Angstroem) = 12.2639/sqrt(hv*(1.+.97845e-06*hv)) hv = high voltage[V, not kV!]
100kV -> 0.0371330 Angstrom
200 -> 0.0250787
300 -> 0.0196870
CL*lambda is the camera constant [CC] in Angstrom*mm, and the scale bar for 1Angstroem**(-1) has the length of CC[mm]
d(Angstrom) = CC/R
I hope my guess concerning the meaning of "2R" was correct.
If you already know what your sample is, you can try free software in link below (diff ring profiler). It uses bitmap images ans you manually calibrate the known rings... otherwise Gerhard said all the important things :D