I used to extract Human Sperm DNA for epigenetic studies.
I have done several protocols but DNA was not free of protein although I used 100 microlitr Proteinase K for 400 microlitr washed semen.
Can anyone guide me or have some hints?
Subhash C. Juneja
It is important forensic point of view. Here are some cites, hope you get your point from these:
https://microgembio.com/product/forensicgem-sperm-dna-extraction-kit/
Also on Google, there are some videos
Videos of extraction of DNA from sperm
Article Genomic DNA Extraction from Sperm
(on researchgate)
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Martiniano Bello
This article may be helpful
Griffin, J. (2013). Methods of sperm DNA extraction for genetic and epigenetic studies. In Spermatogenesis (pp. 379-384). Humana Press, Totowa, NJ.
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