That's an open question. I'm not sure whether you are asking for a solvent to extract compounds from leaves, or a method to partition the extract into different solvents.
I will assume that the bioactive compound is unknown to you. You could extract the leaves with hexane, followed by ethyl acetate, followed by methanol, and finally water. This will extract compounds soluble in different solvent polarities. Others may chime in with other solvents as well, but the ones I mentioned will extract pretty much everything.
If you are asking about which two solvents to partition an existing extract between, it depends on the polarity of your compound and impurities. Solvents pairs that are immiscible include hexane/water, dichloromethane/water, ethyl acetate water, hexane/methanol.
Alkaloids in an extract can be partitioned between acidic water and dichloromethane. Remove the dichloromethane layer and make the water basic. Partition again with fresh dichloromethane and the alkaloids will be in the dichloromethane.
Hope this helps!
Solvent -solvent system means you should use form polar to non polar solvent for better extraction. You can choose any two or a series of solvents from low to high polarity according to your requirement of phytochemicals.
Solvent -solvent system means you should use form polar to non polar solvent for better extraction. You can choose any two or a series of solvents from low to high polarity according to your requirement of phytochemicals.
Initially you use a single sovent - then partition it with another solvent of different polarity - say you ectract the material in slightly acidic water - then partition it with ethyl ether
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