Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

How long does it take for MCF-7 cancer cells to adhere to the microfluidic surface? Is 24 hours enough?

Hello everyone. We have cultured MCF-7 cancer cells in microfluidics several times and incubated them for 24 hours, but as the nanomaterial passed through the cells, the cells moved and no cell...

05 September 2020 7,292 3 View

How can I measure Superoxide dismutase (SOD)?

Hi everyone, SOD measurement need to making assay buffer (Na-PO4 buffer+ NBT, L.Methionine and Riboflavin). I prepare this buffer under dark condition but unfortunately after about 10 -15 min its...

10 September 2018 937 5 View

How can I make DMEM F12 with 30mM glucose concentration for induction of stress oxidative in HUVEC cells?

Hi dear researchers, DMEM F12 glucose concentration is 17.5mM, I added D_glucose in basal cell culture until reached 30mM, but HUVEC cells had better grown than control group after 48 hours. I...

06 April 2018 8,650 1 View

Clustering network vehiculer in ns2?

I need to compare my work with the Reservation-Based (RB)code that described in the "Offloading Floating Car data" article. Can anyone give me the its code? I work with ns2 simulator

06 September 2017 3,631 1 View

What is the longest lifetime reported for electron and holes in perovskite solar cells to date?

When these materials get excited with light, electron and holes are generated but it is important for them to hang arround for a while before they recombine so that more photocurrent will be...

26 August 2017 9,161 11 View

What is the density of poly vinyl alcohol (SB220)?

Density of PVA (SB220)

06 February 2017 8,921 5 View

Why the type of organic solvent can affect particle size of nanoparticles in film hydration method?

For example dichloromethane and chloroform

11 November 2016 1,428 1 View

How to prevent/reduce hysteresis effect in dye sensitized solar cells (DSSCs) / perovskite solar cells?

I have read that one of the major challenges in solar cells applications is reducing Hysteresis. First off, can anyone give an explanation of this phenomenon? Secondly, how to face this...

27 July 2016 8,620 5 View

Can we study Pests and dieback using L-band PALSAR data?

12 July 2016 6,369 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How UAV or drone can be beneficial to better estimate crop yield?

As we know, crop yield estimation has been done traditionally with farmer's expert knowledge, deductive sampling in representative areas, and remote sensing with satellite imagery. Farmer's Expert...

01 March 2021 3,468 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View