How can I detect ATP released from a microbe upon treatment with an antibiotic?

More Ashwini Kumar Dubey's questions See All

How to update the citations of articles at researchgate?

My articles are cited in other articles but it is not updated at researchgate. Does anyone knows to update the citations at researchgate? Thank you.

03 April 2017 6,322 0 View

Original research article of the reaction between silver nitrate and halide ions?

Does anyone have the original research article of the reaction between silver nitrate and halide ions? Thank you! A K Dubey

01 February 2017 746 0 View

How can I avoid smearing of protein bands in tris-tricine SDS-PAGE?

Dear all, My protein of interest is

08 September 2015 4,101 6 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

How to avoid ATP degradation while performing ATP assay?

I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...

15 February 2021 3,746 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View

Why below-ground ecology is still unexplored (majority of it) in our country ?

Below ground ecology or below ground ecological functions and ecosystems roles are very crucial and highly significant for any ecosystem especially for the forest ecosystems. The interaction among...

02 January 2021 6,912 3 View

What are the role of microbes for green revolution or modern advancement in science ?

We all know the role of microbes its miniature of the miracle how it can be , used in revolutionizing science in various fields.

23 December 2020 6,220 10 View

Adam B Shapiro

The luminescence method is the most sensitive one by far for detecting ATP. This means you can use the smallest amount of bacteria and/or the least amount of ATP release. If you want to use another method, you will need much more bacteria. UV absorbance at 260 nm is not specific to ATP.

Arne Lundin

I agree with Adam. Whether ATP or ADP and AMP leaks out depends on the action mechanism of the antibiotic. If you just want to see the leakage I suggest measuring ATP+ADP+AMP continuously by adding the required reagents to the cell suspension. I can provide instructions if you send an email to [email protected].