Dear Charchit,

You can do it by preparing 1mM solution of 1H,1H,2H,2H - perfluorodecane thiol in ethanol (99% pure or better) and immersing your gold coated substrate for at least 2 hours. You can check our paper in Langmuir, 2008, 24 (22), pp 13007–13012 where we used the same protocol to get very hydrophobic mono-layer on flat gold surfaces. 

However, I would recommend you to keep the substrate immersed overnight for a packed layer.

Hope this helps.

Regards

Santanu

Usually the standard protocol for getting maximum surface coverage and best molecule packing is immersion of a freshly prepared or clean substrate (in case of gold substrate, the  pirhana solution can be use to clean it) into a dilute (1-10 mM) ethanolic solution of thiols for 12-18 h at room temperature. 

http://pubs.acs.org/doi/abs/10.1021/cr0300789

And if you prefere not using solvents you can just put your samples in hermetic glass container with small amount of thiol (one droplet will be enough) and put it into oven for a few hours at 60deg.

Hi,

May I ask if any of you knows whether this kind of thiol reacts with metals like Ni?I have a metal sheet ou of Ni used for casting, and I want to do some face treatment of it. Just wondering if thiols can be applied on it.

Thanks!

Thiols should react with Ni also, but for such metals I think that fluorinated phosphonic acids will be better choice.

I am using PEG-Biotinylated thiol to attach on gold surface. Should I use ethanol as solvent or PBS?

@Jatin

No, you don't use PBS. Simply use ethanol and rinse after overnight immersion (minimum two hours). Check our paper on similar PEG-Biotinylated thiol work [Langmuir 2015, 31, 1921−1930], DOI: 10.1021/la503213f

By the way, why you want to use PBS? Any specific reason?

Regards,

Santanu

@Santanu Sir, I read a similar paper regarding biotinylated-Au probe synthesis, http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.6b00529 , where they have used PBS buffer.

Thanks.