How can I conduct acid phosphatase analysis?

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Adam B Shapiro

If you want to measure the catalytic activity of the enzyme, there are some simple spectrophotometric methods. A very easy one is to use p-nitrophenyl phosphate as the substrate. Hydrolysis by the enzyme results in a colored product.

https://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-acid-phosphatase.html