Is there any funded internship opportunity in soil science for 6 months to 1 year abroad India?

I have carried out research on Characterization of soil Arsenic contamination & mitigation options through water management and varietal selection of Rice for my masters dissertation & am...

09 November 2019 5,071 5 View

What could have gone wrong in the 2Dgel where the protein (soluble fraction) seem to have aggregated at low and high pH?

I am new to 2D gel electrophoresis. I tried to follow the basics but the results have come as an absolute shocker. As a newbie my troubleshooting skill is as limited as my hands on skill in the...

20 July 2018 5,140 4 View

No title

16 March 2017 8,085 4 View

How can I increase the expression level of a protein?

I tried to purify a fungal protein using E. coli BL21 DE3 strain. Unfortunately, it has very low expression.

07 December 2014 252 8 View

I am getting a PCR amplification from a brain region, albeit a faint signal, while in RNA in situ I am not. Is this normal?

The signal varies with physiological state and RNA in situ signals are present when the transcription is quite high. The probes are all working and the protocol is fully standardized Is it so that...

21 October 2014 3,602 3 View

Steps for lineament detection using landsat data?

Do I have to use kernels or are there other methods?

03 January 2014 6,786 4 View

How to evaluate plastic epsilon zz during plane stress implementation of Drucker-Prager plasticity model ?

01 January 1970 1,157 4 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View