How can I collect blood from small loach without sacrificing it?

Manik Acharjee @Manik-Acharjee

16 October 2013 0 7K Report

I want to work on immunity of hill-stream loach. Effect of temperature on immune response of hill-stream loach.

More Manik Acharjee's questions See All

Can anyone help me about how to optimize the structure in excited state in gaussian?

Recently, in a research paper, i found that the author had optimized the structures in an S1 excited state (S0 = G.S).

17 December 2023 9,107 8 View

Is there any Post Doctoral position or Research Job related to Environmental Contamination, Waste Management, Soil-Plant-Nutrient cycle available?

Hello All, Greetings! I have been looking for a Post Doctoral position or a Research Job related to Environmental Contamination, Waste Management, Soil-Plant-Nutrient cycle. Please do let me...

25 November 2023 2,911 1 View

What is the XRD peak value of Molybdenum(Mo) single crystal of (100) and (111) orientation?

I need the peak values of (100) and (111) orientation of pure Molybdenum crystal .

21 March 2023 5,660 3 View

Can anyone please provide me JCPDS file for Mo (JCPDS 01-1208)?

I need validation of XRD peaks.

14 March 2023 9,004 3 View

Why the extracted PHB from Pseudomonas lactis comes in semi-viscous form rather than in its powdered form?

Dear Researchers, I'm engaged in research related to the isolation, synthesis, and characterization of bacterial polyhydroxybutyrate (PHB). I've carefully followed solvent extraction method (using...

13 October 2022 6,476 5 View

How can I manage the delay in doing a research due to slow IRB clearance?

As per my knowledge, it is mandatory to have an ethical clearance from an authority, especially from the institutions a researcher works, to perform research. But, for some reason, my IRB has a...

09 May 2020 1,227 1 View

How to implement *STATIC, STABILIZE, factor=2e-4 command in Calculix ???

Dear Readers, The "*STATIC, Stabilize, Factor=2e-4" is commonly used in Abaqus to initiate adaptive automatic damping algorithm. I am looking to implement the same using Calculix but "Stabilize"...

01 April 2020 4,342 0 View

Is there any funded internship opportunity in soil science for 6 months to 1 year abroad India?

I have carried out research on Characterization of soil Arsenic contamination & mitigation options through water management and varietal selection of Rice for my masters dissertation & am...

09 November 2019 5,337 5 View

Is there any book or article/s on interactions and dynamics of different plant nutrients under microgravity?

Plants can play major role in the survival of human beings on long-duration space missions and probably in a mission to Mars and Moon. Plants are known to adapt to extreme ecological conditions on...

06 November 2019 594 5 View

During BrDu immunofluorescence detection why rat brain coronal cryosections fall off soon after the 1N/2N HCl denaturation step gelatin subbed sides?

Currently I am processing BrdU labelled rat brains for immunofluorescent detection. The cryosections are of 25 µm. My concern is that soon after the 2N HCl denaturation step the sections fall off...

05 February 2019 3,026 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View