Hello, everyone. Please help me, recently I'm doing a research on bacterial resistance. I get my results about resistance frequency, for example, my bacteria shows a resistance rate of 76% to penicillin. But I don't now if its a "low" resistance rate, a "moderate" resistance rate, a "high" resistance rate or another category of classification. I search a lot of articles about that topic but I don't get some of them that explain why they categorize some rates as "high" or "low", or if there are a range for that categories. If you can help with articles or information about that I will be very grateful with. Thanks for read me!
Dear Juan Pablo Vaca González
Isolates are classified as resistant, sensitive or intermediate based on the latest CLSI or EUCAST guidelines. This data can then be used to determine whether your isolates are XDR or MDR.
Another approach would be to compare your strain with a known susceptible strain using a series of penicillin dilutions designed to reduce colony formation in a range of 10 to 90 %. The response of the bacteria (colony inhibition) must be converted into probits and the concentration of penicillin expressed as a log to give you a straight line. This approach provides you with 2 important statistics - a 50% response and the slope of the concentration - response line. You can use these statistics to quantify resistance and obtain measures of variance for your strain as well. These numbers can also be compared with a typical therapeutic concentration of penicillin.
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