We performed CRISPR/Cas9 editing and managed to introduce a desired point mutation by Homology Directed Repair in HeLa cells.
We now want to check if there are any off-target mutations caused by the procedure, to ensure that further results with these mutated cells actually result from the point mutation.
What I'm looking for is a useful technique that can be used AFTER the whole mutation procedure has already ended, any suggestions?
Geoff Margison
Unless you think that the off-target mutations will be present in certain genes, you have to sequence the whole genome!
What do other CRISPR officionados do about this?
My approach would be to carry out microarray analysis of expressed mRNAs - at least that reduces the magnitude of the task!
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