Dekel Wainer

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Questions related from Dekel Wainer

Which methods for checking homo/heterozygosity of a presented CRISPR/Cas9 mutation?

We recently introduced a mutation in HeLa cells via CRISPR Homology Direct Repair. We now wish to check if the mutation is homozygous or heterozygous. We have already sequenced the mutated area...

05 May 2018 4,277 4 View

How can I check for off-target CRISPR/Cas9 mutations after the desired mutation has already been completed?

We performed CRISPR/Cas9 editing and managed to introduce a desired point mutation by Homology Directed Repair in HeLa cells. We now want to check if there are any off-target mutations caused by...

05 May 2018 921 3 View