I want to calculate the non-linear absorption coefficient values, so need to calculate the linear absorption coefficient (LAC). I have the UV spectrum of the material. How can I measure LAC from this spectrum?
Thanks Yasemin,
This normally absorption coefficient ε calculation. i have found it for linear absorption coefficient is α= A/l
A = obtained abs value from UV spectrum. or A is the decadic absorbances.
Read more: http://www.answers.com/topic/absorption-coefficient-biochemistry#ixzz36Izh8vLP
You could calculate it simply following the Lambert-Beer law:
starting with the formla: I = Io . 10-epsilon.c.l , from this formula it follows:
I/Io = 10-epsilon.c.l , then Io/I = 10epsilon.c.l and log Io/I = A = epsilon . c. l ,
A is absorbance, epsilon is molar absorption coefficient, c is molar concentrationn of solution and l corrseponds to thickness of layer of solution in quvette.
When you know A from the UV spectrum and c as well as l are known to you, you can calculate absorption coeff. easily.
You should examine the dependence of the absorbance of the solution concentration for the selected wavelength. Using the Lambert-Beer law and linear regression you can calculate the absorption coefficient. For large c the relationship can be nonlinear
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