What could be the possible reason for lack of Mtb protein expression cloned in pET28a even after induction ?

12 December 2020 2,584 8 View

Why the IT services companies (based in India) are mainly patenting only at the USPTO?

Please suggest some papers/reports on the changes in the policy with respect to patenting of computer software in India. Does it have any implications as to why the IT services companies (mostly...

12 November 2020 8,351 1 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Can i use surfactant C-TAB in extraction and detection of the aminaphthalene sulfonates by HPLC technique?

In the detection of Aminonaphthalene sulfonates the quaternary ammonium salts like Tetrabutyl ammonium bromide ,Triethyl ammonium acetates are used so the C-TAB being an quarternary ammonium...

21 September 2020 9,636 1 View

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

Please suggest some good quality and free journal with very short response time for MEMS, piezoelectric energy harvester research?

27 August 2020 994 4 View

What is the critical chloride concentration for concrete made of GGBS and for concrete made with Silica fume ? Is it 0.5% ?

Ccrit or Cth values for concrete made with different mineral admixtures

21 July 2020 7,301 2 View

Objective function for Pulp library in python?

How do we define objective functions while doing optimization using Pulp in Python. We have defined Prediction models separately. Next step is to bring objective functions from prediction models...

06 July 2020 9,256 3 View

How i can dry Metal Nano crystals ?

freeze-drying is recommended for nanocrystals drying but other than this technique direct heat is useful to Metal Nano crytals

17 June 2020 1,238 3 View

I need to predict the time when a physical test on a machine will fail. Which time series forecast/ machine learning method should I use?

There are 2 columns: time(in seconds), amplitude of wave. I need to predict when the test may fail and we can observe a crack on the machine based on the amplitude. I do not know the...

30 May 2020 7,594 13 View

IDT or Geneart, which is best for full lenght gene synthesis?

Did anyone got full lenght genes synthesized from both IDT and Geneart and which one will be the best in terms of pricing, post purchase support and delivery speed. Preferably in India but the...

14 May 2020 942 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View