The following links may be seen:

http://rbms.cnpms.embrapa.br/index.php/ojs/article/view/229/pdf_260

http://www.scielo.br/pdf/asagr/v33n3/v33n3a23.pdf

Gill anf Delouche describe a set of variables as seedling growth rate, in prectice the plant mass after a fixed time from emergence. The comparison can be done with anova or t test, https://www.jstor.org/stable/23432492?seq=1#page_scan_tab_contents

Dear Michelle Christine Chirinos-Arias

- Adjust the moisture content of your seed sample to about 20% in the laboratory according to controlled deterioration test (ISTA, 2010).

- Take 10 sub-samples of 100 seeds from your working sample and place them separately in aluminum foil bags and seal them.  

- Place the bags in an oven at 40 °C.

- Take a bag out of oven every 2 days, and carry out a germination test in four replicates of 25 seeds at 20-25 °C.

- Use the following equation of linear regression to show changes in seed viability during storage at 40 °C (Ellis and Roberts, 1980):

v = Ki – (1/ σ ) p

where v is probit viability, Ki is the intercept or initial viability, p is storage period, and 1/ σ is the slope which is showing the rate of seed deterioration.

Regards

Kazem Ghassemi-Golezani

The deterioration of a seed could be deduced from :

Estimation of the germinative potential over time.

For example, take estimates for fresh seed lot,there after take values every week until results go very low.

Estimates of seed weight over time could also be taken.

Seed deterioration would be noticed either via the status of the embryo or that of the cotyledon.