I have cobalt oxide nanoparticles. How can I calculate the absorption coefficient of my sample? I don't know the thickness. How can I calculate the thickness of cobalt oxide nanopowder?
The absorption coefficient (also known as molar absorptivity or molar extinction coefficient) for UV data can be calculated using the Beer-Lambert law, which relates the absorbance (A), concentration (C), path length (l), and absorption coefficient (ε) as follows:
A=ε⋅C⋅l
A is the absorbance of the sample.
ε is the absorption coefficient (molar absorptivity or molar extinction coefficient) with units of L/(mol·cm) or M⁻¹·cm⁻¹, depending on the concentration units.
C is the concentration of the absorbing species in moles per liter (Molarity, M).
l is the path length through which the light passes in centimeters (cm).it is remain constant 1 cm
To calculate the absorption coefficient (ε), you'll need to rearrange the Beer-Lambert equation as follows:
ε=A/C⋅l
To calculate ε, you need to know the absorbance (A) of your sample at a specific wavelength, the concentration (C) of the absorbing species, and the path length (l) of the sample cell.
The result will have units of L/(mol·cm) or M⁻¹·cm⁻¹, depending on the units of concentration (C).