How can I build a nano transformer?

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Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How can I publish my final master project as an article for a scientif journal?

I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.

16 July 2024 7,431 1 View

How to get "density" and "viscosity" for cavitation(water vapor) for cavitating Francis turbine?

Hello there I am simulating cavitation for a Francis turbine. Francis turbine is rotating with 826 rpm. I have two questions; 1- Vapor pressure for my case is 2100 Pascal, and Temperature is 25...

13 May 2024 4,403 7 View

How to avoid segmentation in Level Set Topology Optimization ( I would need to localize the variation of the level-set)?

Dear All, I am trying to converge on an optimal solution for a compliance minimization problem using a parametric RBF-based level set method. However, I have faced the following problem(s) for...

14 February 2024 9,511 0 View

How to model anchored cable bolts (passive bolts) in FLAC 2D 8.1 software?

I am investigating the impact of pretension effect around circular tunnels. How can I do my own modeling? 1- I want to excavate a tunnel. 2- I want to put a rock bolt or cable bolt in the hole 3-...

08 February 2024 7,166 1 View

What are the boundary conditions for a 2D Channel when we know pressure inlet?

Hello everyone I am working in a 2D channel where I know the inlet Pressure as a function of cosine, and out let pressure at atmospheric level. What should I use for inlet and outlet boundary...

26 November 2023 6,412 5 View

How can I have smooth boundaries in topology optimization using level-set method?

I am working on topology optimization using the parametric level set method. Regardless of the methodology, I thought it might be a generic problem in Topology optimization with which many...

05 October 2023 2,725 4 View

How to prepare a distribution map of Fritillaria genus in the world?

Fritillaria is one of the largest genera of the Liliaceae family. There are about 170 taxa of this genus all over the world. These are mostly native to the temperate regions of the Northern...

03 July 2023 5,678 0 View

Can I use convergence behavior of residuals to predict if my flow is laminar or turbulent when I use steady state simulation?

Hello all, I am trying to predict Drag coefficient of an object(and I am not interested in the vortex shedding after my object, also I cant afford LES), so I decided to use steady state...

05 April 2023 1,357 0 View

Endogenously tagging protein with His tag??

Hi everyone, There are challenges with using antibodies to capture my protein of interest so I will be using CRISPR to endogenously tag my RNA binding protein with a fluorophore followed by a His...

28 March 2023 8,638 1 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

06 August 2024 8,716 0 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Nano Orange protein quant on the Quantus fluorometer?

Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...

05 August 2024 8,946 3 View