How can i avoid or eliminate oxidized molecules from my parietal protein extract?

07 July 2019 1 7K Report

hello all,

these days i am tryingb to extract parietal proteins from Marchantia polymorpha plants but we noticed that this plant is full with oxidized molecules especially when doing protein extraction. the protein extract was dark brownish.

we got an advice to use DTT to prevent oxidation of phenols but nothing change.

so anyone can help us by giving an advice on how to get rid of oxidation during protein extraction?.

thanks in advance.

regards.

Hasan Kolkas

hello,

thanks for your answer and tomorrow i will try your suggestion (ascorbic acid) but the question is at what concentration i should use it ?. is it necessary to add GSH to prevent autooxidation of ascorbic acid ?.

and the protein oxidation was investigated and detected by mass spectrometry method.

thanks

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