How can I assay biofilm forming at air-water interface or in the form of plaque????

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Edison Andres Cruz-Olivo

Dear colleague,

You can use the Calgary Device designed by the University of Calgary. It is used to create biofilms of any bacteria strain. Once you have grown your biofilm, you can test any antimicrobial you want.

Look for this paper: Harrison JJ, Stremick CA, Turner RJ, Allan ND, Olson ME, Ceri H. Microtiter susceptibility testing of microbes growing on pegs lids: a miniaturized biofilm model for high-throughput screening. Nature Protocols; 2010 Vol. 5, No. 7.

Feel free to contact me if you need more information.

Best regards!

Amol Kanampalliwar

thnx sir