Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

During transformation of Agrobacteriumu with plasmids ,PCR Positive colonies are not POSITIVE after restreaking.. Why?

Initially we are getting positive amplification .The positive colonies are selected and restreaked.The PCR is not showing positive amplification.

18 December 2020 9,802 3 View

How to calculate trapping parameter of the deconvolution peaks?

28 July 2020 2,413 2 View

Have any professors ever done research about the expression of fusion proteins in Drosophila S2 cells?

1. How to scale up the expression of fusion proteins? 2. How to improve the purity of fusion protein to 90%? I have tried affinity chromatography, but the purity by SDS-PAGE was not satisfied....

04 November 2019 8,479 6 View

How to calculate the active area of CNTs modified glassy carbon electrode?

"The active area of so porous electrode as the authors used could not be correctly evaluated using the Randles-SEVCIK formula. Just because the depletion layers of active places may overlap." How...

28 October 2019 1,782 3 View

Future of bioinformatic analysis in plant science ?

please share your idea regarding the future of bioinformatic analysis in plant science? recommend the preferred PC and MAC programs for the beginner ,and for the advanced users? as well as any...

24 October 2019 8,814 4 View

Raman cross section?

Can anyone help me to calculate Raman cross section in RE doped glasses?

12 December 2018 6,330 1 View

Which is the best kit/enzymatic method Efficient for the isolation of cells from the mouse or human tumors?

I want to isolate the cells from the tumors (mouse model and human). I read that Collagenase I is harsher than Collagenase IV. Can anyone please suggest if there is any commercial kit with the...

08 August 2018 4,961 2 View

Hi Everyone, can anyone tell how to get growth kinetics of bacteria with the help of Hoechst stain??

Hoechst 33258 stain

15 May 2018 3,128 3 View

Can any one give Jcpds fileof ZnSe and CdZnSe?

i have obtained an additional peak in XRD at 31.7 degree. so i want to get the idea about this peak few articles mentioned it but not showing angle values.

29 November 2017 3,772 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View