Hello,

To convert Area% from an HPLC chromatogram into molar concentration, first, you will need to use a calibration curve created with a standard solution to relate peak area to concentration. Multiply Area% by the total chromatogram area to find the peak area of your compound. Then, apply the calibration equation (e.g., (C =Peak Area/ Slope of the Curve) to calculate the concentration. Finally, adjust for any sample dilution by multiplying by the dilution factor.

Thanks

Ahmed

To convert area% from an HPLC chromatogram to molar concentration, first calculate the absolute peak area by multiplying the area% by the total peak area. Then, use the response factor (RF) to find the molar concentration using the formula: Molar Concentration = Absolute Peak Area / RF. This process allows for accurate quantification of analytes in your sample.

Direct from Area % alone you cannot get molar concentration

You need :

1) Calibration Curve from known concentrations in mM

2) Run Known Concentration by HPLC and Read the Area

3) Make 2 columns in Excel X-axis's= Concentration and Y-axis's= Peak Area

4) By excel : Shade the 2 columns ---Insert--- Scatter-- Add trendline-- linear ---Set Intercept--- Display Equation on Chart---Display R-squared value on Chart

5) Get the equation with the Excel from the graph Peak (Area=m× Concentration +b )

6) Mit Excel go function Slope--- m=Slope(Peak Area, Concentration)

7) Mit Excel go to function intercept ---- b=INTERCEPT (Peak Area, Concentration)

8) Inject the sample and read the area

9) linear equation: Peak Area=m× Concentration +b

10) make this equation by Excel ( Concentration of Sample(mM)=(Area of the sample- b)/ m)