Can someone describe how to measure latent infection period (days), infection frequency, lesion size and lesion area, lesion growth rate and RAULEC for potato leaves inoculated with phytophthora infestans? Assessment period is 12 days.
Check this reference, it describes in M & M all the components you are looking for:
Chacón, M. G., Andrade-Piedra, J. L., Gessler, C. and Forbes, G. A. (2007), Aggressiveness of Phytophthora infestans and phenotypic analysis of resistance in wild Petota accessions in Ecuador. Plant Pathology, 56: 549–561. doi: 10.1111/j.1365-3059.2007.01604.x
In case you want to measure exactly the area of the lesions, you can also try taking pics of them and use an image software for that purpose, e.g. IMAGEJ, that is freely available http://rsbweb.nih.gov/ij/download.html.
Inoculate with counted number of spores on day 0 and wait for first sign of infection to appear. the day first sign of infection appear is the latent period in days. count the number of lesion per surface area with equal intervals of time till lesions stop appearing this will give you infection frequency. for lesion growth rate measure size of lesion (in mm) at equal intervals of time.
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