Hi everyone
I’m having a lot of problems trying to characterize T-regulatory cells as infiltrates in tumors by FAC`s, the main problem is that the fixation permeabilization buffers needed for Foxp3, intracellular staining are killing most of my cells. So I have seen many papers using Foxp3 marker by IHC but how accurate is this approach? Do i have to perform a triple immunofluorescence using CD4, CD25, Foxp3 in my tissue samples? Thanks in advance.
Best.
Hi Carlos
Onno et.al. 2007 Published article interested T-reg cells Marker by Immunohistochemistry technique I hope help you.
In my experience certain cancer cells do express FoxP3. So I would recommend your triple IF approach.
thanks you both for the help. it seems i will have to move into the triple IF approach
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