We have been trying to identify some of our bacterial isolates using the Biolog Identification system. Two of the isolates, which vary in their physiological fingerprints to an extent, have been assigned the same genus and species by the Biolog. Any clues?
You can confirm the species by PCR using primers genus or species specific primers. Or you can analyse your sample by Microbial Identification System, a MALDİ-TOF based system from BRUKER (microfleks) or SHIMADZU. Good luck..
Biolog Identification is based on the oxidation of different carbon sources by bacterial strains, it is quite normal that strains of same species can have different pattern in biolog plates. So, you can't assign species name only on the basis of it, you need to confirm your results with 16S rRNA gene sequencing or FAME or MALDI-TOF (as suggested by Saban).
MALDİ-TOF based Microbial Identification System, or 16S rRNA gene sequencing technique would be best system for identification of bacterial isolates
You may use Biolog System for preliminary identification of your bacterial strains and then use universal primers of 16S rRNA gene for further molecular identification.
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