How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Are there any method to detect implanted stem cells?

Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...

15 December 2020 6,065 3 View

How to characterize the dispersion of aluminum oxide in Al matrix composite which reinforced by alumina during the powder metallurgy?

Now we could characterize the disperion of Al2O3 in AMCs (pure Al reinforced by Al2O3 ) after sintering. But the process of powder metallurgy has a lot of steps, which take long time. The cycle...

08 November 2020 5,244 3 View

Is that possible the filter of flow cytometry was failed so I got those data using FL1 vs FL2 and FL1 vs FL3?

The cells were expressing GFP and also incubated with PI for dead cells detection. This is my first-time using this BD Accuri C6 and I'm not sure if I need to set compensation (I tried to follow...

01 November 2020 8,360 3 View

What if the abbreviations of two technical terms in the manuscript are the same?

I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?

23 June 2020 2,115 5 View

ImzMLConverter installation & running issues?

Hi Everyone, Did anyone have a similar issue when install and run imzMLConverter? I followed the instruction on https://www.cs.bham.ac.uk/~ibs/imzMLConverter/ and used the command it provides....

23 May 2020 3,936 3 View

How to revise survey items based on unexpected EFA results?

Hi, there. A few days ago, I posed a question about factor analysis of survey results, but I left many important details unspecified. HERE is the more detailed one. I develop a theoretical model...

06 May 2020 4,937 10 View

Many cases of identical answers to consectutive questions.How to revise survey items?

Hi, there. I am working on my Ph.D dissertation. I have run a factor analysis of 56 items with maximum likelihood and direct oblimin method. It turns out that only one factor can be extracted...

25 April 2020 6,267 10 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

Small displacement measurement in vacuum?

Hello, I have a question. there is a circular thin foil which vibrates in vacuum, the foil restricted and moves up and down. it is like a diaphragm between two pipes . both sides are in vacuum but...

18 February 2021 8,075 3 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

Functionality on MWCNT can possible without using vacuum oven?

I want to synthesis functional MWCNT from pristine MWCNT using acid treatment but I haven't vacuum oven for drying but I have a normal hot air oven. So may it possible to use hot air oven for...

02 February 2021 8,475 3 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View