Dear Khaled Sekkoum

Fractional distillation is widely used for isolating/separating different essential oil constituents based on their boiling points.

Good luck.

Thank you very much B.R. Rajeswara Rao

Dear Khaled,

Hydrodistillation method is widely used especially for industrial production of essential oil (E.O.) because it is easy and fast. However, in some cases this method doesn't fit the desirable purposes. For example, in the case of Jasmine, the best way to isolate Jasmine E.O. is Enfleurage and Defleurage. Expression and Pelatrice are suitable for citrus fruits. Therefore, you have to take in your account which plant you will proceed with. Also, if you are looking for the high quality E.O., the is apparatus called Supercritical Fluid Extraction using CO2 but it is so expensive and it mostly used in experimental scale.

Good Luck! 

Dear Dr. Emad Shehata

I think the question pertains to isolation of the main component of the essential oil from the essential oil. The question I suppose is not about the methods to isolate the essential oil from the aromatic plant (a question asked by Prof. Vinesh Kumar and a number of methods have been listed by RG members).

This is only a comment and not criticism!

Best wishes and good luck.

Dear Prof. B.R. Rajeswara Rao,

you are totally right. I misunderstood the question. Thanks a lot. 

DEAR KHALED:

THE BEST TECHNIQUE IS SUPERCRITICAL FLUID CHROMATOGRAPHY (SCF). HOWEVER T=YOU NEED TO HAVE THE INSTRUMENT AVAILABLE IN ORDER TO BE ABLE TO SEPARATE AND ISOLATED THE MAJOR COMPONENTS IN THE ESSENTIAL OIL UNDER STUDY.

Thanks for all answers but my question was how can I separate the major component afer E.O extaction with rapid laboratory technics.

Dear Dr. Khaled

You may follow the following link for some more leads:

https://www.researchgate.net/post/How_can_I_purify_individual_components_from_essential_oils

Laboratory level fractional distillation units are available in the market and are operational. As far as the experimental protocol is concerned, it depends on the essential oil, its constituents and their boiling points.

I am attaching a slide available with me.

Good luck.

You can separate the major components of an essential oil by simple column chromatography

I am agree with Dr. B.R. Rajeswara Rao. Fractional distillation is an efficient and economic method for the same.

If that is present more than 50%, can run a column eluting with hexane.