Research is about discovering new things.If you know it will be effective, why do we have to do research.. your goal is to make it effective or add new knowldege so that someone can pick-it up from there. Develop a good hypothesis and conduct thorough lit review, develop good research plan, and start the journey in untrodden path! Hope you got the answer!

Vinotha Vino wrote:

> I need a opinion

Not only bacteria, but also yeast provides substances to break

down cellulose. A link collection (about 50% in English):

http://www.augos.com/diverses/Energiequellen-Zellulose-Abbau.html

Regards,

Joachim

The effectiveness of this process mainly depnds on the amount of the product and the production period. Both are depending on your microbial isolate. Usualy this process can be performed by two microorganisms; the first is a cellulase producing to hydrolysis the cellulose to glucose, and the second is to utilize the glucose and produces biofuel. ABE producing microorganisms are usualy used. ABE refers to Acetone, butanol, ethanol. This point has been studdied in detail, otherwise, you will genetic modify your isolate to enhance either the cellulase production or glucose/ethanol conversion.

I have worked on cellulose material, rather cellulose waste. The efficiency is determined by what method of pre-treatment is done, what strain of bacterium/yeast, temperature and experimental design such as consolidated system or separate system. Try mix and match to get good results. When I did my preliminary work I was able to get just 2-5% v/v.

see DMC and SSFmethod other method also i think this will help you,

Thanks all of you.....

All the best, its very interesting and we were working on this for past 7 years , go ahead and any doubt feel free to ask us

Okay.... Thank you very much for your comment sir,....

the efficiency of the yield is a real bottleneck in this kind of technology; large quantities of raw materials yield a meager amount of fuel. I think the problem partly lies with the solvent tolerance level of the bacteria used that is organic compound toxicity cause suboptimal growth of the bacteria used. try working around it and see if you come up with anything interesting.

Not sure what do you mean by biofuel production using bacteria? Do you mean delignification and saccharification or fermentation?

I am thinking how about using the ionic liquids in order to dissolve lignin material then it can be fermented by yeast. But you should know the character of plant cell walls because it determine the type of ionic liquid

At present, it depends on the cost of the petroleum fuel in your country. In any case, presently used fuels are not going to last long and then biofuel will be the only sustainable solution.

IT is very important field. Lot of commercial production unit are coming up to produced ethanol from cellulosic waste. IOgen, and Novozyme are setting up plants in US and Canada. Join Biofuel digest. It will give you all imformation.

Its a very important field. I calculated the production of ethanol from the paper waste in our town (250000 habitants). Its about 18000 liters per day. You research could have be a big economic relevant.

Hi It is very important field with lot of funding. Apply to Dr. David Levin

Biosystem Engineering

University of Winnipeg, Manitoba, Canada

In reply to Pareveen Kumar, Iogen and Shell shut the proposed bio ethanol from cellulose plant in Manitoba a couple of weeks ago, as it was considered uneconomic compared to petroleum fuels. The company retrenched many scientists. So much for cellulosic ethanol!!!!! A better approach would be algal biofuels, and you would save the forests as resources to ethanol.

I am in agreement with Robert Dekker. The chosen field is a beaten track but it does not mean that one should not work in this area. If the product is cellulosic alcohol,it is not worth because it is not economical. If the waste is plant based, it becomes still more cumbersome because of the involvement of pectin and lignin.

Therefore, scientists' attention is diverted to algal biofuels, be it EtoH/bio-diesel. May be if you are still interested, run a pilot-scale expt. to work out the cost- benefit ratio employing a good quality of inoculum

I too agree that at present none of the biofuel (2nd to 4rth generation) are economical but still we need to have alternative to fossil fuels as one day for sure these are going to deplet. We have come a long way in research of these cellulosic bioethanol, algal biofuels and biohydrogen. All of these are having some or other technical problems. But for research on these at the moment we cannot focus only on economic values as today which are not economical tomorrow can be, when the petroleum prices will hike as it is in the present mode. In India if the government will remove all the subsidies on pteroleum then bioethanol will be in a position to compete. However I also do not recommend the forest to be utilised for bioethanol, sustainable technology have to come from the waste. Volatile fatty acids obtained from the waste could be feasible in the long run for the production of microbial lipids and biohydrogen. I strongly feel one of these can never be able to replace fossilfuels but all of these together should be. As and when and where, whichever of these resources are available should be utilised.

This would be an interesting research, i earlier worked on celluolosic waste using fungi. My advice is that u should 1st do a test-run by trying 2 isolate cellulosic bacteria from d environment and then use it on different cellulosic waste like waste paper. Thank u and wishing u all d best.

your research is going to be so interesting. If your focus is on biological processes, you have a huge field to explore. Bacteria and yeast for biobased molecules is a very specialized field if you want to target new products from biomass polymers such as hemicellulose and cellulose as well as lignin. Remember that currently roughly 10% of oil is used to produce polymers such as plastics. It is also possible to develop biobased alternative to petrobased products. Polylactic acid, a biocompostable plastic and biopolyethilene a recylclable plastic, are both good examples of biobased plastics made from biomass sources (second generation sources are still not being used with this purpose).

If you want a suggestion, I may say that second generation biofuels is currently being intensively investigated all over the world. A bit more investigation is needed for biobased products.

Hope you the best. If something you need, please visit www.cnib.com.ec so you can understand a little bit more the huge field of yeasts and biorefinery that is awaiting for researchers like you.

Thank u for all ur comments.........and advices

Research is about discovering new things.If you know it will be effective, why do we have to do research.. your goal is to make it effective or add new knowldege so that someone can pick-it up from there. Develop a good hypothesis and conduct thorough lit review, develop good research plan, and start the journey in untrodden path! Hope you got the answer!

Hey...

I have previously worked on production of Bioethanol from weeds like Parthenium and Lantana using a combination of different bacterial strains and yeast. As you know, Parthenium & Lantana are very commonly found in India and are toxic too. So, it would be nice if you could include it in your doctoral research, wherein, you are not only producing bioethanol, but, you are also clearing up these unwanted toxic weeds. Think of the employment it could generate in rural areas of our country.

In the preliminary stages, the % of alcohol produced was just above 2. Optimizing the conditions would definitely improve the overall yield.

Good luck with your research.

Sagar

This work already going to bioprocess lab for microbial biotechnology...this work accepted for women young scientist award..now the project is going on here...

Congratulations! Can you summarize the winner project?

Thank you sir .Yes sir, i was summarized the project ...i have attached the review article for the same work...