Sagar Sridhara

3 Questions 21 Answers 0 Followers

Questions related from Sagar Sridhara

Can we selectively promote protein crystallization against salt crystallization without a complete change of the crystallization condition?

Consider a crystallization condition that readily gives salt crystals on addition of protein. Is there a logical way to increase the probability of protein crystallization against the undesired...

03 April 2013 976 3 View

What may be the reasons for the wrong selection of a transformed bacterial cell?

I transformed E.coli (DH5α) cells with a plasmid bearing streptomycin resistance. But, I had wrongly plated them onto a LB+Kan plate. Surprisingly, some colonies grew. It is highly possible that...

26 February 2013 6,875 1 View

Can a protein crystallize after 2 months of its introduction to a particular well condition?

I was recently trying crystallization trials of my protein sample in a 96-well Corning plate. In one well of an experiment, some phase separations emerged after the 4th day of dispensing the...

17 January 2013 9,909 25 View