Hi, I am in the process of constructing an over-expression vector , for which i am in need of pcDNA4.0 empty backbone. Can anyone gift it to my work?

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Amplification of 3'UTR of a gene from cDNA?

I need to amplifying 3'UTR of a gene from cDNA for the reporter assay. In the first round of PCR, I have got the specific amplification at 584 bp. However when i have re-amplified the PCR product...

06 July 2016 7,107 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out?

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...

31 July 2024 781 1 View

How to find a reaction is reversible or irreversible in CV? How to know the number of electrons transfer?

My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...

30 July 2024 4,671 3 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View

How can we improve transfer learning techniques to make models generalize better across different tasks and domains with limited labeled data?

Machine Learning

24 July 2024 2,487 3 View

VOC analysis on GCMS ?

Hi everyone, Can someone explain for me a method to inject VOC collected with Tedlar bag from pyrolysis process and transfer or inject into GCMS. Thanks

22 July 2024 6,398 1 View