I am not an expert in this field, but I am very interested and have researched to find an answer. I received some assistance from tlooto.com for this response. Could you please review the response below to see if it is correct?

Fish egg yolk extraction requires a protocol tailored to their unique properties, which differ significantly from chicken eggs. Here is a refined protocol for fish egg yolk extraction:

This protocol is optimized for the specific characteristics of fish eggs, ensuring efficient extraction and high-quality yolk material for further analyses.

Reference

[1] Kovacs-Nolan, J., & Mine, Y. (2012). Egg yolk antibodies for passive immunity.. Annual review of food science and technology, 3, 163-82 .

[2] Bourin, M., Gautron, J., Berges, M., Attucci, S., Blay, G. L., Labas, V., Nys, Y., & Réhault-Godbert, S. (2011). Antimicrobial potential of egg yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg.. Journal of agricultural and food chemistry, 59 23, 12368-74 .

[3] Ptáček, M., Stádníková, M., Savvulidi, F., & Stádník, L. (2019). Ram Semen Cryopreservation Using Egg Yolk or Egg Yolk-free Extenders: Preliminary Results. Scientia Agriculturae Bohemica, 50, 103 - 96.

[4] Khatimah, K., Yusuf, M., Utami, R. F., Toleng, A., Masturi, Sahiruddin, & Masir, U. (2021). Comparison of chicken-egg yolk and duck-egg yolk in tris-citric acid as extender to maintain the quality of post-thawing Bali bull semen. IOP Conference Series: Earth and Environmental Science, 788.