Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

How can I find the area of contour with magnitude less than specific number?

Hello everyone I have results from fluent and I want to determine area with magnitude less than specific number for example for velocity or TKE. and also how can I determine Max and min diameter...

02 November 2020 5,863 3 View

Why residuals stay at the same range in vof model in Fluent and it doesn't converge?

hi I'm modeling a 3d channel with vof , k-epsilon RNG with non-equilibrium wall functions , for bc I have velocity inlet, pressure outlet and for top of the domain I have used pressure outlet...

21 August 2020 5,926 7 View

How I can obtain the cartesian coordinates of a complex without single crystals for gaussian calculations?

When the single crystals of complex are not formed, how we can obtain cartesian coordinates for Gaussian calculations? We want to predict kind of interaction or coordination of manganese to amino...

18 August 2020 8,146 3 View

I am confused about the editor's request (I had submitted a manuscript and then major revision was requested). Can anybody help me?

I had submitted a manuscript to a journal. After a while, they requested major revisions. The end of their request, they mentioned that: "You will be unable to make your revisions on the...

09 August 2020 5,436 7 View

Is it possible to extract RNA and protein from 96-well plates?

I am doing a knockdown test in 96 well plates and then I need to extract RNA and protein from my cells. I'm not sure that the number of cells in 96 well plates is enough to these extractions....

12 June 2019 5,459 3 View

In SDS-PAGE , how possible to use freez-dry samples for loading in gel electrophoresis?

I freeze fungal mycelia of mushroom. In SDS-PAGE of total protein ,If I freeze mycelium I can used this sample? How should I prepare mycelium for electrophoresis? How should I make it for...

01 October 2018 7,971 5 View

What is the extinction coefficient of pyrogallol at 430nm for calculation of PPO(polyphenol oxidase) activity?

I performed the polyphenol oxidase activity assay from certain mycelia extracts using pyrogallol as susbtrate. I have the absorbance values but am facing the problem in determining the enzyme...

05 August 2018 172 1 View

Many thanks for all your help?

This question has been deleted.

30 March 2018 7,374 5 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

How to improve the adhesion between germanium and silicon monoxide thin film layers using EBEAM evaporation?

I want to deposit multilayer coating of germanium and silicon monoxide on silicon wafer using ebeam evaporation?

21 February 2021 2,141 3 View

How to deal with different types of logarithmic data for meta-analysis?

Hello dear researchers, 1.I want to know how can I compute pooled effect size when we have three types of logarithmic Mean&SD Type 1. Log (x) with base 10 Type 2. Ln (x) Type 3. Just...

18 February 2021 8,214 3 View