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You have to apply Folch method for the precipitation of proteins (1 ml serum/plasma + 1ml CHCl3 + 0.5 ml methanol). After the recovery of organic phase (lower phase: chloroform) it has to be dried under nitrogen flow. Add methanol/BF3 for transmethylation and extract fatty acid methyl esters in n-hexane. Inject 1 microL n-hexane into GC by using FAME column.

If you only have access to a GC, rather than a GC/MS, then you will also need a set of fatty acid standards.  Note that you will also be able to observe GC peaks for polyunsaturated and saturated fatty acids which will also be present.

Hi! There is another usefull site for lipid analysis by gc and gc/ms. 

http://lipidlibrary.aocs.org/Analysis/?navItemNumber=19195

Using GC you could have a problem with MUFA isomers identifications (close retention times and absence of standards). As for me, GC/MS is a better choice. You can use derivatization technique for unambiguous double bound position identification by MS spectra changing in MUFA isomers (dimethyl disulfide derivatization for example). Polar columns are more preferable, as for me (FFAP or HP-88) for separation. Be aware, some MUFA could coeluate with PUFA isomers on some GC columns.

Convert the MUFA to its FAME form and run it through the GC. For comparison with respect to retention times you need standard sample curves.Every column will have different retention times for the same samples and hence you NEED to run it before.

Hi Mookambika,

If you are looking to separate MUFA FAMEs like Oliec/vaccenenic/transvaccenic/palmitoleic/palmitolaidic etc , I would suggest you to use Supelco 2560 cyano polar column. This column is highly specific for separating isomers .Make sure these MUFA are converted to FAME derivatives for GC or GC-MS. GC with FID works good as well .

For isolation of lipids, you can simple treat the serum/plasma with 0.5 N methanolic NaOH followed by treatment with BF3/Methanol. Extract FAME into organic solvents such as hexane/heptane. The conversion is excellent. Hope this tips helps you.

 Please see my articles

1.                  Rafał Bobiński, Monika Mikulska, Hanna Mojska, Michał Simon. Comparison of the fatty acid composition of maternal blood and cord blood of mothers who delivered healthy full-term babies, preterm babies, and full-term small for gestational age infants. Journal Maternal- Fetal and Neonatal Medicine 2013; 26: 96–102.  

Rafał Bobiński, Monika Mikulska, Hanna Mojska, Michał Simon. Comparison of the fatty acid composition of transitional and mature milk of mothers who delivered healthy full-term babies, preterm babies, and full-term small for gestational age infants. European Journal of Clinical Nutrition 2013;67:966-971