I need to know how the colored proteins react with BCA to give the appropriate color which is to be read by the spectrophotometer? I mean I have worked with colorless proteins up till now. How to go about with these colored protein estimation?
I am not working on your project, but:
To estimate the concentration of colored proteins, you can use the BCA (bicinchoninic acid) protein assay, which is a widely used method for the colorimetric detection and quantification of total protein in a solution.The BCA assay involves the development of a purple-colored reaction product by the chelation of BCA molecules with cuprous ions, and this color can be measured by a spectrophotometer at a wavelength of 562 nm
The intensity of the color is proportional to the protein concentration, allowing for the quantification of protein levels.
The BCA assay is highly sensitive, compatible with a wide range of protein samples, and exhibits a broad dynamic range, making it suitable for estimating protein concentrations in solutions containing colored proteins.
By using a standard curve generated from known protein concentrations, you can determine the protein concentration of your samples based on their absorbance values measured by the spectrophotometer.
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