How to get rid of the free GFP signal in an Atg8-GFP tagged yeast saccharomyces cerevisiae?

I am working with yeast saccharomyces cerevisiae -BY4741- strain. I tagged the Atg8 with GFP in this background and whenever I am performing western blot I get a free GFP signal for my WT which...

15 November 2020 8,556 3 View

Hi all! What vehicle do you recommend for IP injection in mice for a DMSO soluble drug?

I have 2 drugs (PTC-209 and GSK343) that are soluble in DMSO (I already made the stock solution, dissolved to a clear solution) and I have to inject them IP to mice for a 2 week treatment course....

17 September 2020 9,107 3 View

Why is Quantum-dot Cellular Automaton (QCA) technology fail to flourish? Specially, Semiconductor and Magnetic QCA for electronic applications?

In 1993, Lent et al. introduced one of the most promising and emerging computational paradigm known as Quantum-dot Cellular Automaton (QCA) computing. Now, it is almost 30 years have been passed,...

06 May 2020 4,955 7 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

Which are the best journals which accept newcomer students for writing reviews on topics related to biomedical engineering ?

I am looking to communicate with a personnel who deals with beginner-level review articles, and want to learn how to write good reviews and the rules and regulations to follow.

06 March 2020 6,068 3 View

In which reason EDTA used in injectables containing proton pomp inhibitors?

We know that EDTA is used by different reasons (chelating agent, support for antimicrobial effectiveness e.g) in pharmaceuticals. Is there any specific reason to prefer EDTA in injectables...

01 December 2019 5,166 3 View

How to get mean age of fluid for a closed system using FLUENT?

Hi everyone, I am working on simulation of stirred tank reactor operating at batch mode. So, there is no fluid inlet-outlet in the system. Can I calculate the local mean age of fluid by using...

18 November 2019 1,142 3 View

How is the length of any scientific document (report, thesis, essay, conference paper, journal article) calculated ?

I wish to know what aspects of these technical documents are included and excluded while making a word count. Specifically, say, a figure caption, references, tables, table footnotes, page...

24 June 2019 9,815 3 View

Hey friends, I have a problem with my primers, is there any way to do PCR with one weak primer?

I have ordered a pair of primers, forward primer is working well, I have checked it by gel, but revers primer has really weak band in gel, and PCR doesn't work. any suggestion?

18 November 2018 5,958 3 View

I would like to simulate a mesh network in NS2. How can I do it? Which 802.11 I need?

Thanks in advance for your replies.

15 February 2015 8,579 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View